Journal: PLoS ONE

Article Title: Ellagitannins from Rubus Berries for the Control of Gastric Inflammation: In Vitro and In Vivo Studies

doi: 10.1371/journal.pone.0071762

Figure Lengend Snippet: Effect of individual compounds sanguiin H-6 and lambertianin C on NF-κB pathway in human gastric epithelial AGS cells.

Article Snippet: The same quantity of total nuclear proteins, measured by the method of Bradford , was used to assess NF-κB nuclear translocation using the NF-κB (p65) transcription factor assay kit (Cayman) followed by spectroscopy (signal read 450 nm, 0.1 s).

Techniques:

Journal: PLoS ONE

Article Title: Ellagitannins from Rubus Berries for the Control of Gastric Inflammation: In Vitro and In Vivo Studies

doi: 10.1371/journal.pone.0071762

Figure Lengend Snippet: AGS cells at confluence were placed in a medium deprived of FCS, and stimulated with 10 ng/ml TNF-α (panel a) or IL-1β (panel b) for 1 hr. AGS cells were plated at the concentration of 1.5 × 10 6 cells/ml in 60-mm plates. NF-κB nuclear translocation was assessed using the NF-κB (p65) transcription factor assay kit (Cayman) followed by spectroscopy (signal read 450 nm, 0.1 s). Inhibition by 5 µM parthenolide used as reference inhibitor was 37% on TNF-α induced NF-κB nuclear translocation, and 40% on IL-1β induced nuclear translocation. Results are the mean ± sd of three experiments in triplicate. * p<0.05, ** p<0.01, ***p<0.001.

Article Snippet: The same quantity of total nuclear proteins, measured by the method of Bradford , was used to assess NF-κB nuclear translocation using the NF-κB (p65) transcription factor assay kit (Cayman) followed by spectroscopy (signal read 450 nm, 0.1 s).

Techniques: Concentration Assay, Translocation Assay, Transcription Factor Assay, Spectroscopy, Inhibition